RNA
Part:BBa_K3249007:Design
Designed by: Omar El-Fahmawi Group: iGEM19_UC_Davis (2019-10-16)
CHO IL1RN sgRNA 1-3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 431
Illegal XbaI site found at 873
Illegal PstI site found at 416
Illegal PstI site found at 858
Illegal PstI site found at 1301 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 416
Illegal PstI site found at 858
Illegal PstI site found at 1301 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 431
Illegal XbaI site found at 873
Illegal PstI site found at 416
Illegal PstI site found at 858
Illegal PstI site found at 1301 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 431
Illegal XbaI site found at 873
Illegal PstI site found at 416
Illegal PstI site found at 858
Illegal PstI site found at 1301 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
NCBI’s gene search was used to find the sequence of the promoter region for IL1RN. Chop Chop (https://chopchop.cbu.uib.no/) was used to design the sgRNA sequence.
Source
Oligos were synthesized by IDT.