RNA

Part:BBa_K3249007:Design

Designed by: Omar El-Fahmawi   Group: iGEM19_UC_Davis   (2019-10-16)


CHO IL1RN sgRNA 1-3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 431
    Illegal XbaI site found at 873
    Illegal PstI site found at 416
    Illegal PstI site found at 858
    Illegal PstI site found at 1301
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 416
    Illegal PstI site found at 858
    Illegal PstI site found at 1301
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 431
    Illegal XbaI site found at 873
    Illegal PstI site found at 416
    Illegal PstI site found at 858
    Illegal PstI site found at 1301
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 431
    Illegal XbaI site found at 873
    Illegal PstI site found at 416
    Illegal PstI site found at 858
    Illegal PstI site found at 1301
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NCBI’s gene search was used to find the sequence of the promoter region for IL1RN. Chop Chop (https://chopchop.cbu.uib.no/) was used to design the sgRNA sequence.

Source

Oligos were synthesized by IDT.

References